Part:BBa_K1970001
XOR gate like repressor of CI repressor expression
This part uses deoxyribozyme ribonucleases to degrade CI repressor mRNA, thereby silencing the gene. The deoxyribozyme ribonucleases are present in two copies, where expression of one is controlled by the lacI promoter and the other by the tetR promoter.
The regulatory system works like an XOR gate: Inducing the regulator with one, and only one, inducer results in silencing of CI mRNA expression. Inducing both lacI and tetR promoters results in the deoxyribozyme ribonucleases binding to each other through designed complimentary regions, stopping their mRNA degrading activities. Lack of induction of either promoter should prevent either deoxyribozyme ribonuclease from being synthesized by the cell, in turn preventing their degradation of the CI repressor.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1374
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 337
Illegal NheI site found at 1589
Illegal SpeI site found at 1374
Illegal NotI site found at 1381 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 615
Illegal XhoI site found at 1867 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1374
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1374
- 1000COMPATIBLE WITH RFC[1000]
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